Article
Analysis of somatic mutations in kappa transgenes.
The Journal of experimental medicine - 1 Jul 1990
Hackett J, Rogerson B J, O'Brien R L, Storb U
Abstract excerpt
We have examined the nature and localization of somatic mutations in three kappa transgenes cloned from IgG-secreting hybridomas. All of the mutations identified were single base substitutions. Mutations were localized to the variable (V) region and its flanking sequences. In every case, the nucl...
Topics
- Animals
- Base Sequence
- Cell Line
- Cloning, Molecular
- DNA Mutational Analysis
- Gene Rearrangement
- Hybridomas
- Immunoglobulin Variable Region
- Mice
- Mice, Transgenic
- Molecular Sequence Data
- Mutation