Mutation pattern of immunoglobulin transgenes is compatible with a model of somatic hypermutation in which targeting of the mutator is linked to the direction of DNA replication.

Abstract excerpt

We have previously demonstrated that B lymphocyte specific somatic mutations are introduced into the variable regions of immunoglobulin kappa transgenes in two independent transgenic mouse lines. The frequency, distribution and nature of these mutations strongly suggest that they arose as a result of the process of somatic hypermutation, which is responsible, in part, for affinity maturation during an immune...

Topics

• Animals
• Base Sequence
• DNA
• DNA Replication
• Genes, Immunoglobulin
• Hybridomas
• Immunoglobulin Variable Region
• Immunoglobulin kappa-Chains
• Mice
• Mice, Transgenic
• Molecular Sequence Data