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Article

Re-engineering an alphoid(tetO)-HAC-based vector to enable high-throughput analyses of gene function.

Nucleic acids research - 1 May 2013

Kononenko Artem V, Lee Nicholas C O, Earnshaw William C, Kouprina Natalay, Larionov Vladimir

Abstract excerpt

Human artificial chromosome (HAC)-based vectors represent an alternative technology for gene delivery and expression with a potential to overcome the problems caused by the use of viral-based vectors. The recently developed alphoid(tetO)-HAC has an advantage over other HAC vectors because it can be easily eliminated from cells by inactivation of the HAC kinetochore via binding of tTS chromatin modifiers to its...

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Identifiers

PMID: 23558748
DOI: 10.1093/nar/gkt205
PMCID: PMC3664798

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