Article
Generation of a conditionally self-eliminating HAC gene delivery vector through incorporation of a tTAVP64 expression cassette.
Nucleic acids research - 19 May 2015
Kononenko Artem V, Lee Nicholas C O, Liskovykh Mikhail, Masumoto Hiroshi, Earnshaw William C, Larionov Vladimir, Kouprina Natalay
Abstract excerpt
Human artificial chromosome (HAC)-based vectors represent an alternative technology for gene delivery and expression with a potential to overcome the problems caused by virus-based vectors. The recently developed alphoid(tetO)-HAC has an advantage over other HAC vectors because it can be easily eliminated from cells by inactivation of the HAC kinetochore via binding of chromatin modifiers, tTA or tTS, to its...
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