Article
Site specific mutation of the Zic2 locus by microinjection of TALEN mRNA in mouse CD1, C3H and C57BL/6J oocytes.
PloS one - 1 Jan 2013
Davies Benjamin, Davies Graham, Preece Christopher, Puliyadi Rathi, Szumska Dorota, Bhattacharya Shoumo
Abstract excerpt
Transcription Activator-Like Effector Nucleases (TALENs) consist of a nuclease domain fused to a DNA binding domain which is engineered to bind to any genomic sequence. These chimeric enzymes can be used to introduce a double strand break at a specific genomic site which then can become the substrate for error-prone non-homologous end joining (NHEJ), generating mutations at the site of cleavage. In this report we...
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