Article
Low antigen dose favours selection of somatic mutants with hallmarks of antibody affinity maturation.
Immunology - 1 Feb 1998
González-Fernández A, Milstein C
Abstract excerpt
The immunization schedule is critical for the derivation of high-affinity antibodies, low antigen dose being particularly favourable for the development of a more efficient memory response. To analyse the molecular events underpinning this preference, we analysed the early maturation of the respo...
Topics
- Animals
- Antibody Affinity
- B-Lymphocytes
- Cell Differentiation
- Clone Cells
- Dose-Response Relationship, Immunologic
- Haptens
- Immunization
- Immunoglobulin Light Chains
- Mice
- Mice, Inbred BALB C
- Mutation
- Oxazolone
- Polymerase Chain Reaction