Article
Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue.
Organic & biomolecular chemistry - 28 Apr 2013
Li Yiming, Pan Man, Li Yitong, Huang Yichao, Guo Qingxiang
Abstract excerpt
Three alkyne-containing pyrrolysine derivatives were synthesized and genetically encoded into proteins by a mutant PylRS-tRNA pair with high efficiencies. With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol-yne ligation reaction.
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