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Article

Genetically encoded unstrained olefins for live cell labeling with tetrazine dyes.

Chemical communications (Cambridge, England) - 7 Nov 2014

Lee Yan-Jiun, Kurra Yadagiri, Yang Yanyan, Torres-Kolbus Jessica, Deiters Alexander, Liu Wenshe R

Abstract excerpt

A number of non-canonical amino acids (NCAAs) with unstrained olefins are genetically encoded using mutant pyrrolysyl-tRNA synthetase-tRNA(Pyl)(CUA) pairs. These NCAAs readily undergo inverse electron-demand Diels-Alder cycloadditions with tetrazine dyes, leading to selective labeling of proteins bearing these NCAAs in live cells.

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Source

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Identifiers

PMID: 25224663
DOI: 10.1039/c4cc06435f
PMCID: PMC4184966

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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