Article
The genetic incorporation of p-azidomethyl-l-phenylalanine into proteins in yeast.
Bioorganic & medicinal chemistry letters - 15 May 2018
Supekova Lubica, Zambaldo Claudio, Choi Seihyun, Lim Reyna, Luo Xiaozhou, Kazane Stephanie A, Young Travis S, Schultz Peter G
Abstract excerpt
The noncanonical amino acid p-azidomethyl-l-phenylalanine can be genetically incorporated into proteins in bacteria, and has been used both as a spectroscopic probe and for the selective modification of proteins by alkynes using click chemistry. Here we report identification of Escherichia coli tyrosyl tRNA synthetase mutants that allow incorporation of p-azidomethyl-l-phenylalanine into proteins in yeast. When...
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