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Article

A rationally designed pyrrolysyl-tRNA synthetase mutant with a broad substrate spectrum.

Journal of the American Chemical Society - 15 Feb 2012

Wang Yane-Shih, Fang Xinqiang, Wallace Ashley L, Wu Bo, Liu Wenshe R

Abstract excerpt

Together with tRNA(CUA)(Pyl), a rationally designed pyrrolysyl-tRNA synthetase mutant N346A/C348A has been successfully used for the genetic incorporation of a variety of phenylalanine derivatives with large para substituents into superfolder green fluorescent protein at an amber mutation site in Escherichia coli. This discovery greatly expands the genetically encoded noncanonical amino acid inventory and opens...

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Identifiers

PMID: 22289053
DOI: 10.1021/ja211972x
PMCID: PMC3288562

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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