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Article

Multiple Click-Selective tRNA Synthetases Expand Mammalian Cell-Specific Proteomics.

Journal of the American Chemical Society - 13 Jun 2018

Yang Andrew C, du Bois Haley, Olsson Niclas, Gate David, Lehallier Benoit, Berdnik Daniela, Brewer Kyle D, Bertozzi Carolyn R, Elias Joshua E, Wyss-Coray Tony

Abstract excerpt

Bioorthogonal tools enable cell-type-specific proteomics, a prerequisite to understanding biological processes in multicellular organisms. Here we report two engineered aminoacyl-tRNA synthetases for mammalian bioorthogonal labeling: a tyrosyl ( ScTyrY43G) and a phenylalanyl ( MmPheT413G) tRNA synthetase that incorporate azide-bearing noncanonical amino acids specifically into the nascent proteomes of host cells....

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Identifiers

PMID: 29775058
DOI: 10.1021/jacs.8b03074
PMCID: PMC6598694

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Source receipt: pubmed, retrieved 7 Sept 2026.

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