Article
Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway.
Nature communications - 24 Jun 2021
Ho Joanne M L, Miller Corwin A, Smith Kathryn A, Mattia Jacob R, Bennett Matthew R
Abstract excerpt
Pyrrolysine (Pyl, O) exists in nature as the 22nd proteinogenic amino acid. Despite being a fundamental building block of proteins, studies of Pyl have been hindered by the difficulty and inefficiency of both its chemical and biological syntheses. Here, we improve Pyl biosynthesis via rational engineering and directed evolution of the entire biosynthetic pathway. To accommodate toxicity of Pyl biosynthetic genes...
Topics
- Bacterial Proteins
- Biosynthetic Pathways
- Coliphages
- Directed Molecular Evolution
- Escherichia coli
- Green Fluorescent Proteins
- Lysine
- Microorganisms, Genetically-Modified
- Mutation
- Operon
- RNA, Transfer
- Recombinant Proteins
- Solubility