Article
Computer analysis of mutations that affect antibody specificity.
Proteins - 1 Jan 1990
Novotny J, Bruccoleri R E, Haber E
Abstract excerpt
The mouse hybridoma cell line 40-150 secretes antibodies with high affinity toward the cardiac glycosides digoxin and digitoxin. A spontaneous mutant, 40-150 A2.4, produces an antibody which carries a single residue mutation, Ser----Arg, in its heavy chain (H94) and has an altered specificity. A...
Topics
- Animals
- Antibody Specificity
- Cell Line
- Electronic Data Processing
- Humans
- Hybridomas
- Hydrogen Bonding
- Immunoglobulins
- Mice
- Mutation
- Protein Conformation