Article
Drastic change in idiotypic but not antigen-binding specificity of an antibody by a single amino-acid substitution.
Nature - 1 Jan 2000
Radbruch A, Zaiss S, Kappen C, Brüggemann M, Beyreuther K, Rajewsky K
Abstract excerpt
In proliferating B lymphocytes, somatic mutation of rearranged antibody variable (V)-region genes occurs at high frequency and may have a key role in the selection of these cells. It is of interest in this context to learn in which way single mutations can affect antigen binding and/or idiotypic specificity of an antibody. Previous investigations have analysed spontaneous mutants of myeloma and hybridoma cells in...
Topics
- Amino Acid Sequence
- Animals
- Antibody Specificity
- Epitopes
- Haptens
- Immunoglobulin Idiotypes
- Immunoglobulin Variable Region
- Immunoglobulin delta-Chains
- Mice
- Mutation
- Nitrohydroxyiodophenylacetate