Experimental analysis by site-directed mutagenesis of somatic mutation effects on affinity and fine specificity in antibodies specific for lysozyme.

Article

Experimental analysis by site-directed mutagenesis of somatic mutation effects on affinity and fine specificity in antibodies specific for lysozyme.

Journal of immunology (Baltimore, Md. : 1950) - 15 Jan 1992

Lavoie T B, Drohan W N, Smith-Gill S J

Abstract excerpt

To experimentally examine the functional roles of somatically derived structural variation in the lysozyme-binding mAb HyHEL-10, we have introduced three different point mutations and one insertion at two different sites in HyHEL-10 by site-directed mutagenesis and expression of the mutant antibo...

Topics

Amino Acid Sequence
Animals
Antibody Affinity
Antibody Specificity
Base Sequence
Molecular Sequence Data
Muramidase
Mutation
Structure-Activity Relationship

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