Article
Contribution of a single heavy chain residue to specificity of an anti-digoxin monoclonal antibody.
Protein science : a publication of the Protein Society - 1 May 1994
Schildbach J F, Shaw S Y, Bruccoleri R E, Haber E, Herzenberg L A, Jager G C, Jeffrey P D, Panka D J, Parks D R, Near R I
Abstract excerpt
Two distinct spontaneous variants of the murine anti-digoxin hybridoma 26-10 were isolated by fluorescence-activated cell sorting for reduced affinity of surface antibody for antigen. Nucleotide and partial amino acid sequencing of the variant antibody variable regions revealed that 1 variant had...
Topics
- Amino Acid Sequence
- Animals
- Antibodies, Monoclonal
- Antibody Specificity
- Base Sequence
- Binding Sites
- Cardenolides
- Cardiac Glycosides
- Crystallography, X-Ray
- DNA
- Digoxin
- Genetic Variation
- Hybridomas
- Immunoglobulin Heavy Chains