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Article

Contribution of a single heavy chain residue to specificity of an anti-digoxin monoclonal antibody.

Protein science : a publication of the Protein Society - 1 May 1994

Schildbach J F, Shaw S Y, Bruccoleri R E, Haber E, Herzenberg L A, Jager G C, Jeffrey P D, Panka D J, Parks D R, Near R I

Abstract excerpt

Two distinct spontaneous variants of the murine anti-digoxin hybridoma 26-10 were isolated by fluorescence-activated cell sorting for reduced affinity of surface antibody for antigen. Nucleotide and partial amino acid sequencing of the variant antibody variable regions revealed that 1 variant had a single amino acid substitution: Lys for Asn at heavy chain position 35. The second variant antibody had 2 heavy...

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Identifiers

PMID: 8061604
DOI: 10.1002/pro.5560030503
PMCID: PMC2142714

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Source receipt: pubmed, retrieved 7 Sept 2026.

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