Article
A spontaneous variant of an antidigoxin hybridoma antibody with increased affinity arises from a heavy chain signal peptide mutation.
Molecular immunology - 1 Apr 1992
Shaw S Y, Margolies M N
Abstract excerpt
The A/J murine hybridoma cell line 40-150 secretes antidigoxin antibodies with high affinity for digoxin. A first-order spontaneous mutant (40-150 A2.4) produces antibodies containing a mutation at heavy chain position 94 resulting in reduced affinity for digoxin. A second-order mutant (40-150 A2...
Topics
- Amino Acid Sequence
- Animals
- Antibody Affinity
- Base Sequence
- Blotting, Southern
- DNA
- Digoxin
- Gene Rearrangement
- Hybridomas
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
- Mice
- Mice, Inbred A
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Protein Sorting Signals
- Sequence Homology, Nucleic Acid