A V region mutation in a phosphocholine-binding monoclonal antibody results in loss of antigen binding.

Abstract excerpt

A V region mutant producing an antibody that had lost the ability to bind phosphocholine was isolated from a hybridoma producing a germline encoded T15 antibody. The mutation resulted in a single aspartic acid to asparagine substitution at residue 95 of the H chain V region. This confirms that the aspartic acid at residue 95 plays a major role in Ag binding. The results also suggest that somatic cell genetic...

Topics

• Amino Acids
• Animals
• Antibodies, Monoclonal
• Base Sequence
• Immunoglobulin Variable Region
• Mice
• Mice, Inbred BALB C
• Molecular Sequence Data
• Mutation
• Phosphorylcholine
• Protein Conformation