Engineering human prolactin to bind to the human growth hormone receptor.

doi:10.1126/science.247.4949.1461

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Article

Engineering human prolactin to bind to the human growth hormone receptor.

Science (New York, N.Y.) - 23 Mar 1990

Cunningham B C, Henner D J, Wells J A

Abstract excerpt

A strategy of iterative site-directed mutagenesis and binding analysis was used to incorporate the receptor-binding determinants from human growth hormone (hGH) into the nonbinding homolog, human prolactin (hPRL). The complementary DNA for hPRL was cloned, expressed in Escherichia coli, and mutat...

Topics

Amino Acid Sequence
Base Sequence
Cloning, Molecular
Growth Hormone
Humans
Molecular Sequence Data
Mutation
Plasmids
Prolactin
Protein Conformation
Receptors, Somatotropin
Recombinant Proteins

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