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Article

Engineering human prolactin to bind to the human growth hormone receptor.

Science (New York, N.Y.) - 23 Mar 1990

Cunningham B C, Henner D J, Wells J A

Abstract excerpt

A strategy of iterative site-directed mutagenesis and binding analysis was used to incorporate the receptor-binding determinants from human growth hormone (hGH) into the nonbinding homolog, human prolactin (hPRL). The complementary DNA for hPRL was cloned, expressed in Escherichia coli, and mutated to introduce sequentially those substitutions from hGH that were predicted by alanine-scanning mutagenesis and other...

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Identifiers

PMID: 2321008
DOI: 10.1126/science.247.4949.1461

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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