Article
The functional binding epitope of a high affinity variant of human growth hormone mapped by shotgun alanine-scanning mutagenesis: insights into the mechanisms responsible for improved affinity.
Journal of molecular biology - 5 Sept 2003
Pal Gabor, Kossiakoff Anthony A, Sidhu Sachdev S
Abstract excerpt
A high-affinity variant of human growth hormone (hGH(v)) contains 15 mutations within site 1 and binds to the hGH receptor (hGHR) approximately 400-fold tighter than does wild-type (wt) hGH (hGH(wt)). We used shotgun scanning combinatorial mutagenesis to dissect the energetic contributions of individual residues within the hGH(v) binding epitope and placed them in context with previously determined structural...
Topics
- Alanine
- Binding Sites
- Epitopes
- Human Growth Hormone
- Humans
- Models, Molecular
- Mutation
- Peptide Library
- Protein Binding
- Protein Conformation
- Receptors, Somatotropin