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Article

Rapid mapping of protein functional epitopes by combinatorial alanine scanning

25 Jul 2000

Abstract excerpt

A combinatorial alanine-scanning strategy was used to determine simultaneously the functional contributions of 19 side chains buried at the interface between human growth hormone and the extracellular domain of its receptor. A phage-displayed protein library was constructed in which the 19 side chains were preferentially allowed to vary only as the wild type or alanine. The library pool was subjected to binding...

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PMID: 10908667
DOI: 10.1073/pnas.160252097

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Source receipt: pubmed, retrieved 7 Sept 2026.

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