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Article

Multiplexed Enzyme Activity-Based Probe Display via Hybridization.

ACS combinatorial science - 9 Nov 2020

Cavett Valerie, Paegel Brian M

Abstract excerpt

Emulsions offer the means to miniaturize and parallelize high-throughput screening but require a robust method to localize activity-based fluorescent probes in each droplet. Multiplexing probes in droplets is impractical, though highly desirable for identifying library members that possess very specific activity. Here, we present multiplexed probe immobilization on library beads for emulsion screening. During...

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Identifiers

PMID: 32803953
DOI: 10.1021/acscombsci.0c00116
PMCID: PMC7655629

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