Article
Premature chromosome condensation is induced by a point mutation in the hamster RCC1 gene.
Molecular and cellular biology - 1 Feb 1990
Uchida S, Sekiguchi T, Nishitani H, Miyauchi K, Ohtsubo M, Nishimoto T
Abstract excerpt
At the nonpermissive temperature, premature chromosome condensation (PCC) occurs in tsBN2 cells derived from the BHK cell line, which can be converted to the Ts+ phenotype by the human RCC1 gene. To prove that the RCC1 gene is the mutant gene in tsBN2 cells, which have RCC1 mRNA and protein of the same sizes as those of BHK cells, RCC1 cDNAs were isolated from BHK and tsBN2 cells and sequenced to search for...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Cycle
- Cell Cycle Proteins
- Cell Line
- Chromosomes
- Cloning, Molecular
- Cricetinae
- DNA
- DNA-Binding Proteins