tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.

doi:10.1016/0006-291x(92)90692-e

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Article

tsBN75 and tsBN423, temperature-sensitive x-linked mutants of the BHK21 cell line, can be complemented by the ubiquitin-activating enzyme E1 cDNA.

Biochemical and biophysical research communications - 30 Apr 1992

Nishitani H, Goto H, Kaneda S, Yamao F, Seno T, Handley P, Schwartz A L, Nishimoto T

Abstract excerpt

tsBN75 and tsBN423 are independently isolated temperature-sensitive (ts) mutants of the BHK21 cell line for cell growth. Both tsBN75 and tsBN423 belong to the same complementation group and show G2 block at the nonpermissive temperature. Both were efficiently transformed to ts+ cells with the mouse and human cDNA encoding the ubiquitin-activating enzyme, E1. While no transformants of tsBN423 cells had a DNA...

Topics

Animals
Cell Division
Cell Line
Cell Line, Transformed
Cricetinae
DNA
Flow Cytometry
Genetic Complementation Test
Humans
Ligases
Mice

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Temperature

Transfection

Ubiquitin-Activating Enzymes

Ubiquitin-Protein Ligases

X Chromosome

Source

Open in PubMed

Identifiers

PMID: 1575720
DOI: 10.1016/0006-291x(92)90692-e

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1575720-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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