Article
Molecular cloning of the human gene, CCG2, that complements the BHK-derived temperature-sensitive cell cycle mutant tsBN63: identity of CCG2 with the human X chromosomal SCAR/RPS4X gene.
Journal of cell science - 1 Sept 1991
Watanabe M, Furuno N, Goebl M, Go M, Miyauchi K, Sekiguchi T, Basilico C, Nishimito T
Abstract excerpt
A temperature-sensitive mutant tsBN63 cell line was isolated by the fluorodeoxyuridine method from the BHK21/13 cell line after mutagenesis with nitrosoguanidine. When cultures of tsBN63 cells growing asynchronously at 33.5 degrees C were shifted to 39.5 degrees C, a nonpermissive temperature, the ability for protein synthesis was rapidly reduced and cell proliferation stopped mainly at G1 phase, and partly at G2...
Topics
- Amino Acid Sequence
- Animals
- Cell Cycle
- Cell Line
- Cloning, Molecular
- DNA
- Genetic Complementation Test
- Humans
- Molecular Sequence Data
- Mutation
- Phenotype