Article
Temperature-sensitive vaccinia virus mutants identify a gene with an essential role in viral replication.
Journal of virology - 1 Feb 1990
Rempel R E, Anderson M K, Evans E, Traktman P
Abstract excerpt
Vaccinia virus mutants ts2 and ts25, members of the same complementation group, exhibit a temperature-dependent arrest at the stage of viral DNA replication. The lesions responsible for the mutant phenotypes have been localized to the far left region of the HindIII B genomic fragment by marker rescue studies. Hybrid selection analyses established that the DNA fragments positive for rescue represented the first...
Topics
- Animals
- Cell Line
- Cosmids
- DNA, Viral
- Genes, Viral
- Kinetics
- L Cells
- Mice
- Molecular Weight
- Mutation
- Nucleic Acid Hybridization
- Plasmids