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Article

Temperature-sensitive mutations in the vaccinia virus H4 gene encoding a component of the virion RNA polymerase.

Journal of virology - 1 Oct 1992

Kane E M, Shuman S

Abstract excerpt

Four previously isolated temperature-sensitive (ts) mutants of vaccinia virus WR (ts1, ts31, ts55, and ts58) comprising a single complementation group (R. C. Condit, A. Motyczka, and G. Spizz, Virology 128:429-443, 1983) have been mapped by marker rescue to the H4L open reading frame located within the genomic HindIII-H DNA fragment. The H4 gene is predicted to encode a 93.6-kDa polypeptide expressed at late...

Topics

Amino Acid Sequence
Blotting, Western
Cells, Cultured
DNA, Viral
DNA-Directed RNA Polymerases
Electrophoresis, Gel, Pulsed-Field
Genes, Viral
Microscopy, Electron
Molecular Sequence Data
Mutation
Temperature

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Transcription, Genetic

Vaccinia virus

Viral Plaque Assay

Virion

Source

Open in PubMed Open full text

Identifiers

PMID: 1527841
DOI: 10.1128/JVI.66.10.5752-5762.1992
PMCID: PMC241450

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1527841-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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