Article
Fine structure mapping and phenotypic analysis of five temperature-sensitive mutations in the second largest subunit of vaccinia virus DNA-dependent RNA polymerase.
Virology - 1 Jan 1990
Hooda-Dhingra U, Patel D D, Pickup D J, Condit R C
Abstract excerpt
We have used plasmid clones spanning the region encoding the 132-kDa subunit of the cowpox virus RNA polymerase (CPV rpo 132) to marker rescue each of five vaccinia virus (VV) temperature sensitive (ts) mutants, ts 27, ts 29, ts 32, ts 47, and ts 62, which together constitute a single complementation group. The experiments fine-map the vaccinia mutations to a 1.3-kb region containing the 3' end of the CPV rpo 132...
Topics
- Animals
- Autoradiography
- Cell Line
- Cowpox virus
- DNA-Directed RNA Polymerases
- Electrophoresis, Polyacrylamide Gel
- Genes, Viral
- Genetic Complementation Test
- Mutation
- Phenotype
- Restriction Mapping