Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins.

doi:10.1128/JVI.66.7.4413-4426.1992

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Article

Vaccinia virus B1 kinase: phenotypic analysis of temperature-sensitive mutants and enzymatic characterization of recombinant proteins.

Journal of virology - 1 Jul 1992

Rempel R E, Traktman P

Abstract excerpt

The vaccinia virus B1 gene encodes a 34-kDa protein with homology to protein kinases. In L cells infected nonpermissively with mutants containing lesions in the B1 gene (ts2 and ts25), the infectious cycle arrests prior to DNA replication. In this report, we demonstrate that DNA synthesis ceases when cultures infected with these mutants at 32 degrees C are shifted to the nonpermissive temperature (39.5 degrees C)...

Topics

Alleles
Animals
Base Sequence
Cell Line
DNA Replication
DNA, Viral
Escherichia coli
L Cells
Mice
Molecular Sequence Data
Mutation
Phenotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Recombinant Proteins

Serine

Substrate Specificity

Temperature

Threonine

Vaccinia virus

Source

Open in PubMed Open full text

Identifiers

PMID: 1602551
DOI: 10.1128/JVI.66.7.4413-4426.1992
PMCID: PMC241249

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1602551-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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