A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution.

doi:10.1128/JVI.65.8.4042-4050.1991

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Article

A temperature-sensitive lesion in the small subunit of the vaccinia virus-encoded mRNA capping enzyme causes a defect in viral telomere resolution.

Journal of virology - 1 Aug 1991

Carpenter M S, DeLange A M

Abstract excerpt

Using pulsed-field gel electrophoresis, we demonstrated that the temperature-sensitive (ts) conditional lethal mutant ts9383 is, at the nonpermissive temperature, defective in the resolution of concatemeric replicative intermediate DNA to linear 185-kb monomeric DNA genomes. The resolution defect was shown to be the result of a partial failure of the mutant virus to convert the replicated form of the viral...

Topics

Animals
Base Sequence
Cell Line
DNA Replication
DNA, Viral
Electrophoresis, Agar Gel
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Viral
Genes, Viral
Methyltransferases
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Multienzyme Complexes

Mutation

Nucleotidyltransferases

Phosphoric Monoester Hydrolases

Restriction Mapping

Temperature

Vaccinia virus

Source

Open in PubMed Open full text

Identifiers

PMID: 1649315
DOI: 10.1128/JVI.65.8.4042-4050.1991
PMCID: PMC248835

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1649315-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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