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Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity.

ACS synthetic biology - 15 Dec 2017

Moreb Eirik Adim, Hoover Benjamin, Yaseen Adam, Valyasevi Nisakorn, Roecker Zoe, Menacho-Melgar Romel, Lynch Michael D

Abstract excerpt

Phage-derived "recombineering" methods are utilized for bacterial genome editing. Recombineering results in a heterogeneous population of modified and unmodified chromosomes, and therefore selection methods, such as CRISPR-Cas9, are required to select for edited clones. Cells can evade CRISPR-Cas-induced cell death through recA-mediated induction of the SOS response. The SOS response increases RecA dependent...

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PMID: 28915012
DOI: 10.1021/acssynbio.7b00174

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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