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Article

Isolation and quantitation of topoisomerase complexes accumulated on Escherichia coli chromosomal DNA.

Antimicrobial agents and chemotherapy - 1 Nov 2012

Aedo Sandra, Tse-Dinh Yuk-Ching

Abstract excerpt

DNA topoisomerases are important targets in anticancer and antibacterial therapy because drugs can initiate cell death by stabilizing the transient covalent topoisomerase-DNA complex. In this study, we employed a method that uses CsCl density gradient centrifugation to separate unbound from DNA-bound GyrA/ParC in Escherichia coli cell lysates after quinolone treatment, allowing antibody detection and quantitation...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 22869559
DOI: 10.1128/AAC.01182-12
PMCID: PMC3486612

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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