Article
Inhibitory activities of quinolones against DNA gyrase and topoisomerase IV purified from Staphylococcus aureus.
Antimicrobial agents and chemotherapy - 1 Nov 1997
Tanaka M, Onodera Y, Uchida Y, Sato K, Hayakawa I
Abstract excerpt
In order to clarify the mechanism of action of quinolones against Staphylococcus aureus, GrlA and GrlB proteins of topoisomerase IV encoded by genes with or without mutations were purified separately as fusion proteins with maltose-binding protein in Escherichia coli. The reconstituted enzymes sh...
Topics
- DNA Topoisomerase IV
- DNA Topoisomerases, Type II
- Microbial Sensitivity Tests
- Mutation
- Polymerase Chain Reaction
- Quinolones
- Staphylococcus aureus