Article
Analysis of topoisomerase function in bacterial replication fork movement: use of DNA microarrays.
Proceedings of the National Academy of Sciences of the United States of America - 15 Aug 2000
Khodursky A B, Peter B J, Schmid M B, DeRisi J, Botstein D, Brown P O, Cozzarelli N R
Abstract excerpt
We used DNA microarrays of the Escherichia coli genome to trace the progression of chromosomal replication forks in synchronized cells. We found that both DNA gyrase and topoisomerase IV (topo IV) promote replication fork progression. When both enzymes were inhibited, the replication fork stopped rapidly. The elongation rate with topo IV alone was 1/3 of normal. Genetic data confirmed and extended these results....
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