Article
Topoisomerase IV, not gyrase, decatenates products of site-specific recombination in Escherichia coli.
Genes & development - 1 Oct 1997
Zechiedrich E L, Khodursky A B, Cozzarelli N R
Abstract excerpt
DNA replication and recombination generate intertwined DNA intermediates that must be decatenated for chromosome segregation to occur. We showed recently that topoisomerase IV (topo IV) is the only important decatenase of DNA replication intermediates in bacteria. Earlier results, however, indica...
Topics
- Bacteriophage lambda
- Blotting, Southern
- DNA Replication
- DNA Topoisomerase IV
- DNA Topoisomerases, Type I
- DNA Topoisomerases, Type II
- DNA, Bacterial
- DNA, Superhelical
- Enzyme Inhibitors
- Escherichia coli
- Integrases
- Kinetics
- Mutation
- Norfloxacin
- Nucleic Acid Conformation
- Plasmids
- Recombinases
- Recombination, Genetic