Translational options for the pir gene of plasmid R6K: multiple forms of the replication initiator protein pi.

doi:10.1016/0378-1119(92)90622-v

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Article

Translational options for the pir gene of plasmid R6K: multiple forms of the replication initiator protein pi.

Gene - 1 Jul 1992

York D, Ivanov V, Gan J, Filutowicz M

Abstract excerpt

The autogenously controlled pir gene of plasmid R6K was believed to encode a single polypeptide that plays multiple roles in the plasmid's biology. We have isolated an opal (op) mutant at the 18th codon of the pir coding frame which does not totally abolish translation of pir mRNA. In extracts of...

Topics

Bacterial Proteins
Base Sequence
Blotting, Western
Carrier Proteins
Codon
Escherichia coli
Escherichia coli Proteins
Genes, Bacterial
Lipoproteins
Molecular Sequence Data
Mutation
Plasmids
Protein Biosynthesis
Recombinant Fusion Proteins

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