Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.

doi:10.1093/nar/20.4.811

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Article

Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.

Nucleic acids research - 25 Feb 1992

Wu F, Goldberg I, Filutowicz M

Abstract excerpt

A dnaA 'null' strain could not support replication of intact plasmid R6K or derivatives containing combinations of its three replication origins (alpha, gamma, beta). DnaA binds in vitro to sites in two functionally distinct segments of the central gamma origin. The 277-bp core segment is common...

Topics

Bacterial Proteins
Base Sequence
Blotting, Western
Carrier Proteins
DNA Helicases
DNA Replication
DNA-Binding Proteins
Enhancer Elements, Genetic
Escherichia coli
Escherichia coli Proteins
Lipoproteins
Molecular Sequence Data
Mutation
Plasmids
Trans-Activators

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