Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.

doi:10.1093/nar/22.20.4211

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Article

Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.

Nucleic acids research - 11 Oct 1994

Filutowicz M, York D, Levchenko I

Abstract excerpt

The replication initiator protein pi of plasmid R6K binds seven 22 bp direct repeats (DR) in the gamma origin. The pi protein also binds to an inverted repeat (IR) in the operator of its own gene, pir, which lies outside the gamma origin sequences. A genetic system was devised to select for pi pr...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
DNA
DNA Helicases
DNA Replication
DNA-Binding Proteins
Molecular Sequence Data
Mutation
Plasmids
Repetitive Sequences, Nucleic Acid
Structure-Activity Relationship
Trans-Activators

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