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Article

Cooperative binding of initiator protein to replication origin conferred by single amino acid substitution.

Nucleic acids research - 11 Oct 1994

Filutowicz M, York D, Levchenko I

Abstract excerpt

The replication initiator protein pi of plasmid R6K binds seven 22 bp direct repeats (DR) in the gamma origin. The pi protein also binds to an inverted repeat (IR) in the operator of its own gene, pir, which lies outside the gamma origin sequences. A genetic system was devised to select for pi protein mutants which discriminate between IR and DR (York et al., Gene (Amst.) 116, 7-12, 1992; York and Filutowicz, J....

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Identifiers

PMID: 7937147
DOI: 10.1093/nar/22.20.4211
PMCID: PMC331923

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