Article
Screening for catalytically active Type II restriction endonucleases using segregation-induced methylation deficiency.
Nucleic acids research - 1 Oct 2012
Ukanis Mindaugas, Sapranauskas Rimantas, Lubys Arvydas
Abstract excerpt
Type II restriction endonucleases (REases) are one of the basic tools of recombinant DNA technology. They also serve as models for elucidation of mechanisms for both site-specific DNA recognition and cleavage by proteins. However, isolation of catalytically active mutants from their libraries is challenging due to the toxicity of REases in the absence of protecting methylation, and techniques explored so far had...
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