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Article

In vivo elimination of parental clones in general and site-directed mutagenesis.

Journal of immunological methods - 1 Feb 2015

Holland Erika G, Acca Felicity E, Belanger Kristina M, Bylo Mary E, Kay Brian K, Weiner Michael P, Kiss Margaret M

Abstract excerpt

The Eco29k I restriction endonuclease is a Sac II isoschizomer that recognizes the sequence 5'-CCGCGG-3' and is encoded, along with the Eco29k I methylase, in the Escherichia coli strain 29k. We have expressed the Eco29k I restriction-methylation system (RM2) in E. coli strain TG1 to produce the strain AXE688. We have developed a directed molecular evolution (DME) mutagenesis method that uses Eco29k I to restrict...

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Share to Cloning, Molecular
Share to Deoxyribonucleases, Type II Site-Specific

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Directed Molecular Evolution

Share to DNA Modification Methylases

Share to DNA, Recombinant

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Share to Mutagenesis, Site-Directed

Source

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Identifiers

PMID: 25523926
DOI: 10.1016/j.jim.2014.12.008
PMCID: PMC4394739

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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