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Article

Isolation of BsoBI restriction endonuclease variants with altered substrate specificity.

Journal of molecular biology - 4 Jul 2003

Zhu Zhenyu, Zhou Jing, Friedman Alan M, Xu Shuang-Yong

Abstract excerpt

BsoBI is a thermophilic restriction endonuclease that cleaves the degenerate DNA sequence C/PyCGPuG (where/=the cleavage site and Py=C or T, Pu=A or G). In the BsoBI-DNA co-crystal structure the D246 residue makes a water-mediated hydrogen bond to N6 of the degenerate base adenine and was propose...

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Source

Identifiers

PMID: 12823974
DOI: 10.1016/s0022-2836(03)00595-3

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026. Prepared record publication-12823974-2026-09-01.1. Manifest publication-graph-cohort-2026-09-01.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 1 Sept 2026.

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