Quantification of thioguanine-resistant lymphocytes from mice irradiated in vivo.

doi:10.1289/ehp.9088129

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Article

Quantification of thioguanine-resistant lymphocytes from mice irradiated in vivo.

Environmental health perspectives - 1 Aug 1990

Lorenz R, Göllner T, Hempel K

Abstract excerpt

Adult mice were Co-60 gamma irradiated, and 7 months later splenocytes were isolated, cultured in microwells, and the frequency of hprt-deficient mutants was determined by measuring the cloning efficiency in media with 6-thioguanine. The mutant frequency at 2, 4, and 6 Gy was 1.6 x 10(-5), 4.4 x...

Topics

Animals
Clone Cells
Drug Resistance
Gamma Rays
Hypoxanthine Phosphoribosyltransferase
Leukocyte Count
Lymphocytes
Mice
Mutagenicity Tests
Mutation
Thioguanine

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