Article
Pseudo-templated transcription by Escherichia coli RNA polymerase at a mutant promoter.
Genes & development - 1 Oct 1990
Jacques J P, Susskind M M
Abstract excerpt
A G----T mutation at the start-point of transcription of the phage P22 sar promoter (sar + 1T) causes a novel defect in promoter clearance by Escherichia coli RNA polymerase (RNAP) in vitro. Under standard transcription conditions, in the presence of high concentrations of all four NTPs, the pred...
Topics
- Base Sequence
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Plasmids
- Poly U
- Promoter Regions, Genetic
- RNA, Bacterial
- Rifampin
- Templates, Genetic
- Transcription, Genetic