Article
Reiterative copying by E.coli RNA polymerase during transcription initiation of mutant pBR322 tet promoters.
Nucleic acids research - 11 Feb 1990
Harley C B, Lawrie J, Boyer H W, Hedgpeth J
Abstract excerpt
The major in vitro transcripts from the tet promoter of pBR322 derivatives pTA22 and pTA33 have heterogeneous 5' ends consisting of variable lengths of oligo(A). Their structure is 5'pppAnU..., where n ranges from 1 to greater than 12, but the template strand can encode at most four A residues at...
Topics
- Base Sequence
- DNA-Directed RNA Polymerases
- Escherichia coli
- Models, Genetic
- Molecular Sequence Data
- Multigene Family
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Transcription, Genetic