Article
Genetic dissection of the transcription cycle. A mutant RNA polymerase that cannot hold onto a promoter.
The Journal of biological chemistry - 5 Oct 1992
Martin E, Sagitov V, Burova E, Nikiforov V, Goldfarb A
Abstract excerpt
Deletion of 10 amino acids from a conserved motif in the beta subunit of Escherichia coli RNA polymerase (RNAP) leads to an interrupted transcription cycle and lethal phenotype. RNAP carrying the mutant subunit retains catalytic function and specificity of promoter recognition but is unable to efficiently hold onto DNA in the binary complex, resulting in a diminished initiation frequency. However, inefficient...
Topics
- Amino Acid Sequence
- Base Sequence
- DNA
- DNA-Directed RNA Polymerases
- Escherichia coli
- Genes, Lethal
- Molecular Sequence Data
- Phenotype
- Promoter Regions, Genetic
- Templates, Genetic
- Transcription, Genetic