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Article

Contribution of partial charge interactions and base stacking to the efficiency of primer extension at and beyond abasic sites in DNA.

Biochemistry - 19 Jun 2012

Xia Shuangluo, Vashishtha Ashwani, Bulkley David, Eom Soo Hyun, Wang Jimin, Konigsberg William H

Abstract excerpt

During DNA synthesis, base stacking and Watson-Crick (WC) hydrogen bonding increase the stability of nascent base pairs when they are in a ternary complex. To evaluate the contribution of base stacking to the incorporation efficiency of dNTPs when a DNA polymerase encounters an abasic site, we varied the penultimate base pairs (PBs) adjacent to the abasic site using all 16 possible combinations. We then...

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Identifiers

PMID: 22630605
DOI: 10.1021/bi300296q
PMCID: PMC3426629

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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