Directed-evolution mutations enhance DNA-binding affinity and protein stability of the adenine base editor ABE8e.

Abstract excerpt

Adenine base editors (ABEs), consisting of CRISPR Cas nickase and deaminase, can chemically convert the A:T base pair to G:C. ABE8e, an evolved variant of the base editor ABE7.10, contains eight directed evolution mutations in its deaminase TadA8e that significantly increase its base editing activity. However, the functional implications of these mutations remain unclear. Here, we combined molecular dynamics (MD)...

Topics

• DNA
• Mutation
• Molecular Dynamics Simulation
• Directed Molecular Evolution
• Gene Editing
• Adenine
• Protein Stability
• Protein Binding
• Static Electricity
• CRISPR-Cas Systems