Article
HhaI DNA methyltransferase uses the protruding Gln237 for active flipping of its target cytosine.
Structure (London, England : 1993) - 1 Jun 2004
Daujotyte Dalia, Serva Saulius, Vilkaitis Giedrius, Merkiene Egle, Venclovas Ceslovas, Klimasauskas Saulius
Abstract excerpt
Access to a nucleotide by its rotation out of the DNA helix (base flipping) is used by numerous DNA modification and repair enzymes. Despite extensive studies of the paradigm HhaI methyltransferase, initial events leading to base flipping remained elusive. Here we demonstrate that the replacement of the target C:G pair with the 2-aminopurine:T pair in the DNA or shortening of the side chain of Gln237 in the...
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