Article
Mutations that alter the ability of the Escherichia coli cyclic AMP receptor protein to activate transcription.
Nucleic acids research - 25 Dec 1990
Bell A, Gaston K, Williams R, Chapman K, Kolb A, Buc H, Minchin S, Williams J, Busby S
Abstract excerpt
The effects of a number of mutations in the E. coli cyclic AMP receptor protein (CRP) have been determined by monitoring the in vivo expression and in vitro open complex formation at two semi-synthetic promoters that are totally CRP-dependent. At one promoter the CRP-binding site is centered around 41.5 base pairs upstream from the transcription start whilst at the other promoter it is 61.5 base pairs upstream....
Topics
- Amino Acid Sequence
- Base Sequence
- Escherichia coli
- Gene Expression Regulation, Bacterial
- Molecular Sequence Data
- Mutation
- Plasmids
- Promoter Regions, Genetic
- Receptors, Cyclic AMP
- Transcription, Genetic