Article
Transcription activation at promoters carrying tandem DNA sites for the Escherichia coli cyclic AMP receptor protein: organisation of the RNA polymerase alpha subunits.
Journal of molecular biology - 10 Apr 1998
Belyaeva T A, Rhodius V A, Webster C L, Busby S J
Abstract excerpt
We have constructed a family of promoters carrying tandem DNA sites for the Escherichia coli cyclic AMP receptor protein (CRP), with one of the sites centred between base-pairs 41 and 42 upstream from the transcription start site, and the second site located further upstream. In vivo activity mea...
Topics
- Base Sequence
- Binding Sites
- Cyclic AMP Receptor Protein
- DNA Footprinting
- DNA Primers
- DNA, Bacterial
- DNA-Directed RNA Polymerases
- Escherichia coli
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Promoter Regions, Genetic
- Protein Conformation
- Transcriptional Activation